Mutate-and-chemical-shift-fingerprint (MCSF) to characterize excited states in RNA using NMR spectroscopy

Magdalena Riad, Noah Hopkins, Lorenzo Baronti, Hampus Karlsson, Judith Schlagnitweit, Katja Petzold

Published: 2021-10-03 DOI: 10.1038/s41596-021-00606-1

NMR spectroscopy

RNA excited states

MCSF method

R1ρ relaxation dispersion

SIRT1 mRNA

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It is important to understand the dynamics and higher energy structures of RNA, called excited states, to achieve better understanding of RNA function. R1ρ relaxation dispersion NMR spectroscopy (RD) determines chemical shift differences between t
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Mutate-and-chemical-shift-fingerprint (MCSF) to characterize excited states in RNA using NMR spectroscopy

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